injectable estradiol valerate, as a substitute for estradiol pellets in breast cancer animal model
نویسندگان
چکیده
the ability to maintain and study human tissues in in-vivo environment has proved to be a valuable tool in breast cancer research for several decades. the most widely tissues have been xenografts established human breast cancer cell lines into athymic nude mice. the aim of this study was to provide a new accurate and affordable method for the establishment of breast cancer xenograftin nude mice. injectable estradiol valerate was assayed as a substitute for estradiol pellets that are rather expensive in order to create cancer tumors by mcf7 xenograft method in nude mice (b6). twenty four healthy adult female nude mice (b6) were injected with different concentrations of pre-counted mcf7 cells. then estradiol valerate and matrigel b.d. were injected either alone or combined in two different groups of animals. in a period of 6 to 9 weeks, mice injected with increased amount of mcf7 cells, estradiol valerate and matrigel (combined or alone) developed faster and larger tumors than animals which received mcf7 only or mcf7 and matrigel combined. the results indicate that estradiol valerate which is way less expensive than estradiol pellets can be used as tumor proliferator to create animal breast cancer models.
منابع مشابه
Injectable Estradiol Valerate, as a Substitute for Estradiol Pellets in Breast Cancer Animal Model
The ability to maintain and study human tissues in in-vivo environment has proved to be a valuable tool in breast cancer research for several decades. The most widely tissues have been xenografts established human breast cancer cell lines into athymic nude mice. The aim of this study was to provide a new accurate and affordable method for the establishment of breast cancer xenograftin nude mice...
متن کاملInjectable Estradiol Valerate, as a Substitute for Estradiol Pellets in Breast Cancer Animal Model
cancer research for several decades. The most widely tissues have been xenografts established human breast cancer cell lines into athymic nude mice. The aim of this study was to provide a new accurate and affordable method for the establishment of breast cancer xenograftin nude mice. Injectable estradiol valerate was assayed as a substitute for estradiol pellets that are rather expensive in ord...
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We read with great interest the Commentary of Griesinger and Kolibianakis (2012) about scheduling oocyte retrieval in GnRH antagonist cycles. Recently, the results of a randomized trial by our group were published, reporting a significant decrease in oocyte retrieval during weekend days following administration of estradiol valerate in the luteal phase of the cycle prior to the stimulated cycle...
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614 P&T® • November 2010 • Vol. 35 No. 1
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Estradiol (E2) regulates gene expression at the transcriptional level by functioning as a ligand for estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta). E2-inducible proteins c-Myc and E2Fs are required for optimal ERalpha activity and secondary estrogen responses, respectively. We show that E2 induces 21 microRNAs and represses seven microRNAs in MCF-7 breast cancer cells; t...
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Most combination oral contraceptives contain ethinyl estradiol and a progestin. A new and novel oral contraceptive formulation combines estradiol valerate (E2V) with dienogest (DNG) in a four-phase dosing regimen. 17β-estradiol is a naturally-occurring estrogen, and a contraceptive pill containing such an estrogen offers potential benefits with regard to metabolic side effects and adverse event...
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مجله بین المللی زیست و زیست پزشکیجلد ۱، شماره ۱، صفحات ۳۵-۳۸
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